goat anti il22ra antibody (R&D Systems)
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goat anti il22ra antibody
Goat Anti Il22ra Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+human+il+22/Human+IL-22+R+alpha+1+Antibody/pm41932922-326-10-14
Average 93 stars, based on 17 article reviews
Goat Anti Il22ra Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+human+il+22/Human+IL-22+R+alpha+1+Antibody/pm41932922-326-10-14
Average 93 stars, based on 17 article reviews
goat anti il22ra antibody - by Bioz Stars,
2026-09
93/100 stars
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Staining:Article Title: Human TYK2 deficiency: Mycobacterial and viral infections without hyper-IgE syndrome Article Snippet: We took aliquots of 10 6 nonadherent cells/ml and either left them unstimulated or stimulated them with 40 ng/ml PMA (Sigma-Aldrich) + 10 −5 M ionomycin (Sigma-Aldrich) for 12 h. All cells were treated with 1 μl/ml GolgiPlug for the final 12 h of culture. .. Surface staining was performed with PE-Cy5–conjugated anti–human CD3 (PE Biosciences) Ab, and intracellular staining was performed with Alexa Fluor 488–conjugated anti–human IL-17 (eBioscience) and PE-conjugated anti–human IFN-γ (BD) or PE-conjugated Article Title: High frequency of intestinal T H 17 cells correlates with microbiota alterations and disease activity in multiple sclerosis Article Snippet: Intestinal cells and PBMCs were assessed for viability by Fixable Viability Dye eFluor 506 (eBioscience) and stained with APC-Cy7 (allophycocyanin-Cy7)–conjugated anti-human CD3 (clone SP34-2, BD Biosciences), PE (phycoerythrin)/Texas Red–conjugated anti-human CD4 (clone S3.5, Thermo Fisher), and Alexa Fluor 700–conjugated anti-human CD8 (clone OKT8, eBioscience) monoclonal antibodies. .. To stain for intracellular cytokines, cells were first stained with the monoclonal antibodies against surface molecules, fixed and permeabilized (BD Cytofix/Cytoperm solution kit, BD Biosciences), and then stained with eFluor 450–conjugated anti-human IFN-γ (clone 4S.B3, eBioscience), APC-conjugated anti-human IL-17A (clone eBio64DEC17, eBioscience), and PE-conjugated Article Title: CD4+ T cells are required to contain early extrathoracic TB dissemination and sustain multi-effector functions of CD8+ T and CD3− lymphocytes Article Snippet: Lung tissues were minced with sharp scissors and squeezed with sterile copper mesh in Petri dish, and suspensions were filtered through 40-um cell strainers and further purified by Ficoll-Paque plus density gradient centrifugation .. The following Abs were used for culture or surface and intracellular cytokine staining for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-PECY7 (SP34-2, BD), CD3-PB (SP34-2, BD), CD4-APC (L200, BD, used for measuring CD4 expression after CD4 depleting Ab treatment), CD8-PB (RPA-T8, BD), CD8-APC (RPA-T8, BD), CD8-PE (RPA-T8, BD), IFN-γ-Allophycocyanin (4S.B3, BD), IFN-γ-PE (4S.B3, BD), TNF-α-PE (MAb11, BD), TNF-α-APC (MAb11, BD), TNF-α-PB (MAb11, eBioscience), IL-17-PE (eBio64CAP17, eBioscience), IL-17-Alexa647 (eBio64CAP17, eBioscience), IL-22-biotinylated ( Article Title: Adjunctive Zoledronate + IL-2 administrations enhance anti-tuberculosis Vγ2Vδ2 T-effector populations, and improve treatment outcome of multidrug-resistant tuberculosis 1 Article Snippet: .. The following Abs were used for culture or surface and intracellular cytokine staining for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-Pacific blue (SP34-2,BD), CD4-BV510 (L200, BD), CD8- Pacific blue (RPA-T8, BD), IFN-γ-APC (4S.B3, BD), IFN-γ Brilliant Violet 711 (4S.B3, Biolegend), TNF-α-PE (Mab11, BD), TNF-α-PE-Cy7 (Mab11, BD), IL-17-PE (eBio64CAP17, eBioscience), IL-22-biotinylated ( Article Title: Multidrug-resistant tuberculosis (MDR-TB) strain infection in macaques results in high bacilli burdens in airways, driving broad innate/adaptive immune responses Article Snippet: .. The following antibodies were used for culturing or surface and intracellular cytokine staining (ICS) for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-PB (SP34-2, BD), CD4-BV510 (L200, BD), CD8-PB (RPA-T8, BD), IFN-γ-APC (4S.B3, BD), IFN-γ Brilliant Violet 711 (4S.B3, Biolegend), TNF-α-PE (MAb11, BD), TNF-α-PE-Cy7 (MAb11, BD), IL-17-PE (eBio64CAP17, eBioscience), IL-22-biotinylated ( Bioprocessing:Article Title: High frequency of intestinal T H 17 cells correlates with microbiota alterations and disease activity in multiple sclerosis Article Snippet: Intestinal cells and PBMCs were assessed for viability by Fixable Viability Dye eFluor 506 (eBioscience) and stained with APC-Cy7 (allophycocyanin-Cy7)–conjugated anti-human CD3 (clone SP34-2, BD Biosciences), PE (phycoerythrin)/Texas Red–conjugated anti-human CD4 (clone S3.5, Thermo Fisher), and Alexa Fluor 700–conjugated anti-human CD8 (clone OKT8, eBioscience) monoclonal antibodies. .. To stain for intracellular cytokines, cells were first stained with the monoclonal antibodies against surface molecules, fixed and permeabilized (BD Cytofix/Cytoperm solution kit, BD Biosciences), and then stained with eFluor 450–conjugated anti-human IFN-γ (clone 4S.B3, eBioscience), APC-conjugated anti-human IL-17A (clone eBio64DEC17, eBioscience), and PE-conjugated Flow Cytometry:Article Title: CD4+ T cells are required to contain early extrathoracic TB dissemination and sustain multi-effector functions of CD8+ T and CD3− lymphocytes Article Snippet: Lung tissues were minced with sharp scissors and squeezed with sterile copper mesh in Petri dish, and suspensions were filtered through 40-um cell strainers and further purified by Ficoll-Paque plus density gradient centrifugation .. The following Abs were used for culture or surface and intracellular cytokine staining for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-PECY7 (SP34-2, BD), CD3-PB (SP34-2, BD), CD4-APC (L200, BD, used for measuring CD4 expression after CD4 depleting Ab treatment), CD8-PB (RPA-T8, BD), CD8-APC (RPA-T8, BD), CD8-PE (RPA-T8, BD), IFN-γ-Allophycocyanin (4S.B3, BD), IFN-γ-PE (4S.B3, BD), TNF-α-PE (MAb11, BD), TNF-α-APC (MAb11, BD), TNF-α-PB (MAb11, eBioscience), IL-17-PE (eBio64CAP17, eBioscience), IL-17-Alexa647 (eBio64CAP17, eBioscience), IL-22-biotinylated ( Article Title: Adjunctive Zoledronate + IL-2 administrations enhance anti-tuberculosis Vγ2Vδ2 T-effector populations, and improve treatment outcome of multidrug-resistant tuberculosis 1 Article Snippet: .. The following Abs were used for culture or surface and intracellular cytokine staining for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-Pacific blue (SP34-2,BD), CD4-BV510 (L200, BD), CD8- Pacific blue (RPA-T8, BD), IFN-γ-APC (4S.B3, BD), IFN-γ Brilliant Violet 711 (4S.B3, Biolegend), TNF-α-PE (Mab11, BD), TNF-α-PE-Cy7 (Mab11, BD), IL-17-PE (eBio64CAP17, eBioscience), IL-22-biotinylated ( Article Title: Multidrug-resistant tuberculosis (MDR-TB) strain infection in macaques results in high bacilli burdens in airways, driving broad innate/adaptive immune responses Article Snippet: .. The following antibodies were used for culturing or surface and intracellular cytokine staining (ICS) for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-PB (SP34-2, BD), CD4-BV510 (L200, BD), CD8-PB (RPA-T8, BD), IFN-γ-APC (4S.B3, BD), IFN-γ Brilliant Violet 711 (4S.B3, Biolegend), TNF-α-PE (MAb11, BD), TNF-α-PE-Cy7 (MAb11, BD), IL-17-PE (eBio64CAP17, eBioscience), IL-22-biotinylated ( Expressing:Article Title: CD4+ T cells are required to contain early extrathoracic TB dissemination and sustain multi-effector functions of CD8+ T and CD3− lymphocytes Article Snippet: Lung tissues were minced with sharp scissors and squeezed with sterile copper mesh in Petri dish, and suspensions were filtered through 40-um cell strainers and further purified by Ficoll-Paque plus density gradient centrifugation .. The following Abs were used for culture or surface and intracellular cytokine staining for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-PECY7 (SP34-2, BD), CD3-PB (SP34-2, BD), CD4-APC (L200, BD, used for measuring CD4 expression after CD4 depleting Ab treatment), CD8-PB (RPA-T8, BD), CD8-APC (RPA-T8, BD), CD8-PE (RPA-T8, BD), IFN-γ-Allophycocyanin (4S.B3, BD), IFN-γ-PE (4S.B3, BD), TNF-α-PE (MAb11, BD), TNF-α-APC (MAb11, BD), TNF-α-PB (MAb11, eBioscience), IL-17-PE (eBio64CAP17, eBioscience), IL-17-Alexa647 (eBio64CAP17, eBioscience), IL-22-biotinylated ( Recombinase Polymerase Amplification:Article Title: CD4+ T cells are required to contain early extrathoracic TB dissemination and sustain multi-effector functions of CD8+ T and CD3− lymphocytes Article Snippet: Lung tissues were minced with sharp scissors and squeezed with sterile copper mesh in Petri dish, and suspensions were filtered through 40-um cell strainers and further purified by Ficoll-Paque plus density gradient centrifugation .. The following Abs were used for culture or surface and intracellular cytokine staining for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-PECY7 (SP34-2, BD), CD3-PB (SP34-2, BD), CD4-APC (L200, BD, used for measuring CD4 expression after CD4 depleting Ab treatment), CD8-PB (RPA-T8, BD), CD8-APC (RPA-T8, BD), CD8-PE (RPA-T8, BD), IFN-γ-Allophycocyanin (4S.B3, BD), IFN-γ-PE (4S.B3, BD), TNF-α-PE (MAb11, BD), TNF-α-APC (MAb11, BD), TNF-α-PB (MAb11, eBioscience), IL-17-PE (eBio64CAP17, eBioscience), IL-17-Alexa647 (eBio64CAP17, eBioscience), IL-22-biotinylated ( Article Title: Adjunctive Zoledronate + IL-2 administrations enhance anti-tuberculosis Vγ2Vδ2 T-effector populations, and improve treatment outcome of multidrug-resistant tuberculosis 1 Article Snippet: .. The following Abs were used for culture or surface and intracellular cytokine staining for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-Pacific blue (SP34-2,BD), CD4-BV510 (L200, BD), CD8- Pacific blue (RPA-T8, BD), IFN-γ-APC (4S.B3, BD), IFN-γ Brilliant Violet 711 (4S.B3, Biolegend), TNF-α-PE (Mab11, BD), TNF-α-PE-Cy7 (Mab11, BD), IL-17-PE (eBio64CAP17, eBioscience), IL-22-biotinylated ( Article Title: Multidrug-resistant tuberculosis (MDR-TB) strain infection in macaques results in high bacilli burdens in airways, driving broad innate/adaptive immune responses Article Snippet: .. The following antibodies were used for culturing or surface and intracellular cytokine staining (ICS) for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-PB (SP34-2, BD), CD4-BV510 (L200, BD), CD8-PB (RPA-T8, BD), IFN-γ-APC (4S.B3, BD), IFN-γ Brilliant Violet 711 (4S.B3, Biolegend), TNF-α-PE (MAb11, BD), TNF-α-PE-Cy7 (MAb11, BD), IL-17-PE (eBio64CAP17, eBioscience), IL-22-biotinylated ( Incubation:Article Title: Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis Article Snippet: The cells were then washed twice with 2% FBS in cold PBS, fixed by incubation with 100 μl Cytofix for 30 min on ice, and washed twice with Cytoperm (Cytofix/Cytoperm Plus fixation/permeabilization kit; BD). .. The cells were then incubated for 1 h on ice with Alexa Fluor 488–conjugated anti–human IL-17A (eBioscience), IL-17F PE-conjugated Enzyme-linked Immunosorbent Assay:Article Title: Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis Article Snippet: The cells were then washed twice with 2% FBS in cold PBS, fixed by incubation with 100 μl Cytofix for 30 min on ice, and washed twice with Cytoperm (Cytofix/Cytoperm Plus fixation/permeabilization kit; BD). .. The cells were then incubated for 1 h on ice with Alexa Fluor 488–conjugated anti–human IL-17A (eBioscience), IL-17F PE-conjugated |
